Personal information:
Name: Marina Gorbatyuk
Primary Department: Vision Science, School of Optometry
Secondary Department: Department of Neurobiology
Business Address: 1670 University Blvd., Birmingham, Alabama 35294
Business phone: (205) 934-6462 (office)
(205) 934-6464 (lab)
e-mail: mgortk@uab.edu
Education:
in Biotechnology Russian Institute of Biotechnology, Moscow, Russia
Postgraduate training
1999-2001 Department of Biological Science, Columbia University, Morningside
Research assistant Campus, New York, NY. Mentor - Alex Tzagoloff, Ph.D.
University of Florida, Gainesville, Florida
Postdoctoral fellow Laboratory of Dr. Alfred Lewin
Academic Appointment:
2012-current Associate Professor, Department of Vision Sciences, of Alabama at
Birmingham.
2012-current Associate Professor, Department of Neurobiology, of Alabama at
Birmingham.
2012-current Associate Scientist, Vision Science Research Center, of Alabama at Birmingham.
2012-current Associate Scientist, Civitan International Research Center, of Alabama at .
2010-2012 Assistant Professor, Department of Cell Biology and Anatomy, University of North Texas Health Science Center.
2004-2010 Research Assistant Professor, Department of Molecular Genetics and Microbiology College of Medicine, University of Florida.
HONORS AND AWARDS:
2006 Young Investigator Travel Award, XII International Symposium on Retinal Degeneration, Argentina.
2011 Board of Directors Award from Foundation Fighting Blindness for “Innovative breakthrough in the development of therapy”.
2012 Young Investigator Travel Award, XV International Symposium on Retinal Degeneration, Germany.
2012 Invited Expert Panel Member for Investigational New Drug Project,
Foundation Fighting Blindness.
2013 Early Career Reviewer (ECR) program, the NIH Center for Scientific Review.
2015-present Elected Biochemistry (BI) Program Committee Member, Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting Program
Teaching Experience:
Undergraduate Courses:
2013-current Science and Technology Honors Program, 4 students
Mentor of 4 students
Graduate Courses:
2015-current Vision Science Graduate Program,
Mentor of 4 students,
VS745, Biology Pathology of the Posterior Segment
Lecturer, “Unfolded Protein Response”
Professional courses:
2014-current VS112, “Physiology of the eye”
Society Memberships:
2001–Present Association for Research in Vision and Ophthalmology (ARVO).
2004-Present American Society of Gene Therapy.
2014- Present International Society for Eye Research.
Invited Reviewer:
2009-current Molecular Therapy
Molecular Vision
PLoSOne
Trends in Molecular Medicine
Progress in retinal and eye research
Investigative Ophthalmology & Visual Science
Professional Membership and Other experience
2012 Foundation Fighting Blindness, ad hoc reviewer.
2013 Editorial Board Member, Journal of Genetic Syndrome & Gene Therapy.
2013 Editorial Board Member, Journal of Cell Science &Therapy.
2013 Editorial Board Member, Molecular Vision.
Administrative Service:
1. Member of the Vision Sciences Faculty Advisory Committee
2. Member of the Vision Science Graduate Program Admissions Committee.
3. Vision Science Research Center (VSRC) Advisory
4. National Eye Institute (NEI) Core Grant Advisory Committee.
Invited Symposium
1. “Modulation of the Unfolded Protein Response in ADRP Retina: Potential Therapeutic Implication”. UCSD, July 25, 2014.
2. “Modulation of the Unfolded Protein Response in ADRP Retina: Potential Therapeutic Implication”. International Society for Eye Research, July 20-24, 2014.
3. “Unfolded Protein Response as a Therapeutic Target for Retinitis Pigmentosa”. UAB Comprehensive Neuroscience Center Retreat, September 5-6, 2013.
4. “Modulation of the rate of retinal degeneration in T17M RHO mice by reprogramming the Unfolded Protein Response”, Association for Research in Vision and Ophthalmology, Annual Meeting, May 4- 9, 2013.
5. “Reprogramming of the downstream UPR markers prevents vision loss in T17M Rho transgenic mice”. XV International Retinal Degeneration Meeting, Bad Gögging, Bavaria, Germany, 2012.
6. “Unfolded Protein Response as a therapeutic target for Autosomal Dominant Retinitis Pigmentosa”. Milwaukee Medical College, March 2012.
7. Protein Response as a therapeutic target for Autosomal Dominant Retinitis Pigmentosa”. Vanderbilt University, Department of Ophthalmology, April 2012.
8. “Unfolded Protein Response as a therapeutic target for Autosomal Dominant Retinitis Pigmentosa”. of Illinois at Chicago, Department of Ophthalmology, May 2012.
9. ”Unfolded Protein Response as a therapeutic target for Autosomal Dominant Retinitis Pigmentosa”. Boston University, Department of Ophthalmology, May 2012.
10. “Unfolded Protein Response as a therapeutic target for Autosomal Dominant Retinitis Pigmentosa”. of North Carolina, Department of Ophthalmology, June 2012.
11. “Unfolded Protein Response as a therapeutic target for Autosomal Dominant Retinitis Pigmentosa”. University of Oklahoma Health Science Center, Department of Ophthalmology, June 2012.
12. “Endoplasmic Reticulum Stress in Retinal Degeneration of P23H Rhodopsin Transgenic Rats” AOPT 10th scientific meeting, Fort Worth, February 2011.
13. “Activation of the Unfolded Protein Response in a Rat Model of ADRP”. Association for Research in Vision and Ophthalmology, Annual Meeting, 2010.
14. “Rescue vision in ADRP animal model by over-expression of GRP78. American Society of Gene
Therapy 11th annual meeting”, 2009.
15. “Gene therapy for mouse models of ADRP”. XIIth International Symposium on Retinal Degeneration, October 23-28, San Carlos de Bariloche, Argentina, 2006.
Selected peer-reviewed publications (in chronological order).
1. Krasnopolskaya, LM, Gorbatyuk, MS, Mozshina, IA, Muromtzev, GS “Comparative studies of Fusicocccum Amygdali Del. Strains – the of , the plant growth regulator”. Mikologiya I Fitopatologiya, Vol. 27, Issue 1, Pages: 46-50, 1993.
2. I., von Kleist-Retzow J.C., Barrientos A., Gorbatyuk M, Taanman JW, Mehaye B, Rustin, P, Tzagoloff A. Munnich, A., . A. “A mutation in the human heme farnesyltransferase gene (COX10) causes cytochrome c oxidase deficiency”. Hum Mol Genet., 9: 1245-1249, 2000.
3. de Lonlay P., I., Barrientos A; Gorbatyuk M, Tzagoloff A, Taanman, JW., Benayoun, E. Chretien D., Kadhom N, A, de Baulny HO, Niaudet P, Munnich A, Rustin P, , A. “A mutant mitochondrial respiratory chain assembly protein causes complex III deficiency in patients with tubulopathy, encephalopathy liver failure”. Nat Genet., 29:57-60, 2001.
4. Gorbatyuk, M.S., Pang, J.-J., Thomas Jr., J., Hauswirth, W.W., Lewin, A.S. “Knockdown of wild-type mouse rhodopsin using an AAV vectored ribozyme as part of an RNA replacement approach”. Mol Vis., 11, 648-656, 2005.
5. Gorbatyuk, M.S., Justilien, V., Liu J., Hauswirth W.W., Lewin A.S. “Preservation of photoreceptor morphology and function in P23H rats using an allele independent ribozyme”. Exp Eye Res., 84: 44-52, 2007.
6. Gorbatyuk M.S., Justilien V., Liu J., Hauswirth W.W., Lewin A.S. “Suppression of mouse rhodopsin expression in vivo by AAV mediated siRNA delivery”. Vision Res., 47:1202-8, 2007.
7. Han Z., Gorbatyuk M.S., Thomas, Jr., J., Lewin, A.S. Srivastava, A. and Stacpoole, P.W. “Down-regulation of expression of rat pyruvate dehydrogenase E1α gene by self-complementary -associated virus-mediated small interfering RNA delivery”. Mitochondrion, 7:253-9, 2007.
8. Gorbatyuk M, Hauswirth W. and Lewin A. “Gene therapy for mouse models of Autosomal Dominant Retinitis Pigmentosa “ in Retinal Degenerative Diseases. Adv Exp Med Biol., Vol. 613, 2007.
9. Gorbatyuk, M.S., Knox, T., LaVail, M.M., Gorbatyuk, O.S., Noorwez, Hauswirth, W.W., Lin, JH, Muzyczka, N. Lewin, AS “Restoration of Visual Function in P23H Rhodopsin Transgenic Rats by Gene Delivery of BiP/Grp78”. Proc Natl Acad Sci USA., 107:5961-6, 2010.
10. Gorbatyuk, O.S, Li. S, Nash, K, Gorbatyuk, M.S., Lewin, AS, Sullivan LF, Mandel, RJ, Chen, Meyers, CW, Manfredsson, FP, and Muzyczka, N. “In Vivo RNAi-Mediated α-Synuclein Silencing Induces Nigrostriatal Degeneration, Mol Ther., 7:1327-37, 2012.
11. Haoyu Mao, Thomas James, Alison Schwein, Arseniy E. Shabashvili, William W. Hauswirth, Marina S. Gorbatyuk and Alfred S. Lewin “AAV delivery of wild-type rhodopsin preserves retinal function in a mouse model of autosomal dominant retinitis pigmentosa”. Hum Gene Ther., 5: 567-75, 2011.
12. Marina S. Gorbatyuk, Oleg S. Gorbatyuk, Matthew M. LaVail, Jonathan H. Lin, William W. Hauswirth and Alfred Lewin “Functional Rescue of P23H Rhodopsin Photoreceptors by Gene delivery” in Retinal Degenerative Diseases. Adv Exp Med Biol., Volume 723, 2011.
13. Haoyu Mao, Marina S. Gorbatyuk, William W. Hauswirth, and Alfred S. Lewin “Gene delivery of wild-type rhodopsin rescues retinal function in an autosomal dominant retinitis pigmentosa mouse model” in Retinal Degenerative Diseases. Adv Exp Med Biol., Volume 723, 2011
14. Gorbatyuk M, Shabashvili A., Chen W, Meyers G, Sullivan L., Lin J., Lewin A., Muzyczka N. and Gorbatyuk O. “Glucose regulated protein 78 (GRP78/BiP) meliorates alpha-synuclein (α-syn) neurotoxicity in a rat model of Parkinson disease". Mol Ther., 7:1327-37, 2012.
15. Mao H, Gorbatyuk M., Rossmiller B., Hauswirth W., and Lewin A. “Long Term Rescue of Retinal
Structure and Function By Rhodopsin RNA Replacement With a Single AAV Vector in P23H RHO”. Transgenic Mice”. Hum Gene Ther., 4:356-66, 2012.
16. Shinde V., Sizova O., Lin J., LaVail M. and Gorbatyuk M. “ER stress in retinal degeneration in S334ter Rho rat” One, 3:e33266, 2012.
17. Deng WT., Dinculescu A., Li Q., Boye S., Li J., Gorbatyuk M., Pang J-j., Chiodo V., Liu L., Alkuraya F., Zhang K., Vollrath D., LaVail M., Hauswirth W. “Tyrosine-mutant AAV8 delivery of human MERTK provides long-term retinal preservation in RCS Rats”. Invest Ophthalmol Vis Sci., 4: 1895-904, 2012.
18. Kunte MM, Choudhury S, Manheim JF, Shinde VM, Miura M, Chiodo VA, Hauswirth WW, Gorbatyuk OS, Gorbatyuk M.S. “ER stress is involved in T17M rhodopsin-induced retinal degeneration”. Invest Ophthalmol Vis Sci., 7:3792-800, 2012.
19. Kroeger H, C., Ahern K., Gee J., Joseph V., Matthes M., Yasumura D, Gorbatyuk M., Chiang W, LaVail M. and Lin J. “Induction of Endoplasmic Reticulum Stress Genes, BiP and Chop, in Genetic and Environmental Models of Retinal Degeneration”. Invest Ophthalmol Vis Sci., 9;53(12):7590-9. : 10.1167/iovs.12-10221, 2012.
20. Shreyasi Choudhury, Yogesh Bhootada and Marina Gorbatyuk “Caspase-7 Ablation Modulates UPR, Reprograms TRAF2-JNK Apoptosis and Protects T17M Rhodopsin Mice from Severe Retinal Degeneration”. Cell Death Dis., 7;4:e528. : 10.1038/cddis.2013.34, 2013.
21. Sonali S. , Alfred S. Lewin and Marina Gorbatyuk “Ablation of C/EBP Homologous Protein Does Not Protect T17M RHO Mice from Retinal Degeneration”. PLoS One, 8(4):e63205, : 10.1371/journal.pone.0063205, 2013.
22. Marina Gorbatyuk and Oleg Gorbatyuk “The Molecular Chaperone GRP78/BiP as a Therapeutic Target for Neurodegenerative Disorders”. Review. Jour of Gen Syndr & Gen The., 4(2), 2013.
23. Marina Gorbatyuk and Oleg Gorbatyuk. “ Review: Retinal Degeneration: Focus on UPR”. Mol Vis., 19:1985-1998 <http://www.molvis.org/molvis/v19/1985. 2013.
24. Xiaoqin Wang, Guibo Wang, Mansi Kunte, Vishal Shinde and Marina Gorbatyuk. “ Modulation of Angiogenesis by Genetic Manipulation of ATF4 in Mouse Model of Oxygen Induced Retinopathy”.
Invest Ophthalmol Vis Sci., 54(9):5995-6002. : 10.1167/iovs.13-12117, 2013.
25. Olga S. Sizova, Vishal M. Shinde, Austin Lenox and Marina S. Gorbatyuk “Modulation of Cellular Signaling Pathways in P23H Rhodopsin Photoreceptors”. Cell Signal., 4:665-72. : 10.1016/j.cellsig.2013.12.008. Dec 27, 2014.
26. Tapasi Rana, Vishal Shinde, Christopher Starr, Artem Kruglov, Evan Boitet, Pravallika Kotla, Sergei Zolotukhin, Alecia Gross and Marina Gorbatyuk “An activated unfolded protein response promotes retinal degeneration and triggers an inflammatory response in the mouse retina” Cell Death and Disease (2014) 5, e1578; :10.1038/cddis.2014.
27. Shreyasi Choudhury, Sonali , Yogesh Bhootada, Mansi Motiwale Kunte, Oleg Gorbatyuk, Alfred S. Lewin, Marina Gorbatyuk “Modulation of the rate of retinal degeneration in T17M RHO mice by reprogramming the Unfolded Protein Response” in Retinal Degenerative Diseases. Adv Exp Med Biol., : 10.1007/978-1-4614-3209-8-58, Vol.801, 2014.
28. Anne R. Murray, Linda Vuong, Daniel Brobst, Steven J. Fliesler, Neal S.Peachey, Marina S. Gorbatyuk, Muna Naash Muayyad R. Al-. “Glycosylation of rhodopsin is necessary for its stability and incorporation into photoreceptor outer segment discs” Human Molecular Genetics, 2015, Vol. 24, No. 10.
29. Maxim Salganik, Valeriy G. Sergeyev, Vishal Shinde, Craig A. Meyers, Marina S. Gorbatyuk, Jonathan H. Lin, Sergey Zolotukhin, Oleg S. Gorbatyuk “The loss of glucose-regulated protein 78 (GRP78) during normal aging or from siRNA knockdown augments human alpha-synuclein (a-syn) toxicity to rat nigral neurons” Neurobiology of Aging, 36 (2015) 2213-2223.
30. Chiang WC, Joseph V, Yasumura D, Matthes MT, Lewin AS, Gorbatyuk MS, LaVail MM, Lin JH. 2015. Ablation of Chop transiently enhances photoreceptor survival but does not prevent retinal degeneration in transgenic mice expressing human P23H rhodopsin. (in review, Adv ExpMed Biol.)
Book chapters
1. JJ Fritz JJ, M.S. Gorbatyuk, A.S. Lewin, W.W. Hauswirth. “Design and validation of therapeutical hammerhead ribozymes for autosomal dominant diseases”. Methods Mol Biol., 252:221-36 2004.
Ongoing research funding:
1. RO1 NIH 1R01EY020905-01, Gorbatyuk M., Role (PI).
“Unfolded Protein Response as a Therapeutic Target for ADRP Animal Models”. The major goal of this project is to study cellular mechanisms involved in ADRP photoreceptor death and develop gene therapy based on the blockage of apoptosis in ADRP photoreceptors. 09/30/2010 – 06/30/2015.
Completed:
1. Foundation Fighting Blindness, Gorbatyuk M., Role (PI).
“Gene Therapy for Autosomal Dominant Retinitis Pigmentosa Based on the Over-expression of Molecular Chaperone GRP78” - TA-GT—0409-0508NTERI.
The major goals of this project are to support research studying the mechanism of Autosomal Dominant Retinitis Pigmentosa in transgenic rats and design the therapeutic treatment for retinal degeneration.
2. Hope for vision, Gorbatyuk M., Role (PI).
“Anti-apoptotic Gene Therapy for ADRP rat model”. The major goal of this project is to support research leading to of anti-apoptotic gene therapy for ADRP rat model.